National Repository of Grey Literature 7 records found  Search took 0.00 seconds. 
Aktivace mitochondriální glycerol-3-fosfátdehydrogenázy různými analogy koenzymu Q
Rauchová, Hana ; Mikšík, Ivan ; Fato, R. ; Bergamini, C. ; Lenaz, G.
The aim of our study was to test the synthetic analogues of coenzyme Q (CoQ) on the activity of mitochondrial glycerol-3-phosphate dehydrogenase. We used idebenone and its two derivatives: methoxyidebenone and acetylidebenone and three other componds in which aliphatic chain of CoQ was substituted by thiazoimidazol type groups. The present results can support our previous data suggesting differences between mitochondrial glycerol-3-phosphate dehydrogenase and other dehydrogenases of respiratory chain in the transfer of reducing equivalents to the CoQ pool
Konstrukce kmenů Corynebacterium glutamicum s nadprodukcí valinu
Elišáková, Veronika ; Holátko, Jiří ; Pátek, Miroslav
Promoters of genes involved in biosynthesis of branched-chain amino acids in Corynebacterium glutamicum were mutagenized. The promoters with increased or decreased activities were used for construction of novel valine-overproducing strains
Plasmidové vektory pro genové manipulace u Rhodococcus erythropolis
Veselý, Martin ; Knoppová, Monika ; Pátek, Miroslav
Vectors for rhodococci, based on plasmids from corynebacteria, were constructed. They are used for gene cloning and analysis of gene expression in phenol-degrading rhodococci.
Charakterizace klonů odvozených od kolorektální nádorově buněčné linie HT29
Šloncová, Eva ; Kučerová, Dana ; Vojtěchová, Martina ; Turečková, Jolana ; Tuháčková, Zdena ; Sovová, Vlasta
During our analysis of HT29 cell line we established several clones, which differed in the expression of some molecular markers, e.g. phosphorylation of Src protein, PKCbeta2 activity, CEA-CAM1 expression and in the response to the differentiating effect of sodium butyrate, indicated by activity of alkaline phosphatase
Využití afinitní chromatografie pro isolaci rostlinných bílkovin vážících steroly
Kamlar, Marek ; Macek, Tomáš ; Kohout, Ladislav
Here we report on the identification of one protein isolated from tobacco callus during affinity chromatography on columns with immobilised brassinosteroids
Charakterizace ječmenných proteinů a glukoproteinů pomocí kombinace separačních metod a hmotnostní spektrometrie
Štikarovská, Markéta ; Chmelík, Josef
This work deals with the combination of separation methods with MALDI-TOF MS. Barley proteins and glycoproteins were characterizated
Analýza produktů degradace škrobu a jiných karbohydrátů ze smrkového jehličí použitím různých analytických technik
Cabálková, J. ; Chmelík, Josef
Starch degradation products were analysed by AsFlFFF-MALS-RI and SEC-MALS-RI for determination of molar mass distribution. Separation of low molecular weight fractions of carbohydrates using HPLC-RI led to the identification of various mono-, di-, tri and tetrasaccharides. MALDI-TOF MS spectra showed the presence of higher oligosaccharides

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